Journal: Signal Transduction and Targeted Therapy

Article Title: Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease

doi: 10.1038/s41392-023-01437-0

Figure Lengend Snippet: Albumosomes interact with CPT2 in the cytoplasm. a Experimental design model. WT mice were fed by the normal or Western diet for 8 weeks before their livers were harvested ( n = 3). b Screening flow of the results of Co-IP and MS assays ( n = 3). c Volcano plots of proteins in the MS result, anti-albumin vs. isotype control. The proteins that have more than 50% difference between the two groups with p < 0.05 were considered to be potential candidates. d , e Proteins that meet the criteria were classified by cellular localization. f Western blot validation of the interaction between albumin and Cpt2 in livers. Representative images were shown. Relative intensity was measured from western blot results. g Western blot results that show the interaction between endogenous albumin and endogenous CPT2 in HepG2. Representative images were shown ( n = 3). h Western blot results that show the interaction between transfected albumin and endogenous CPT2 in HEK293T. Representative images were shown. Relative intensity was measured from western blot results ( n = 2). i , j Confocal microscopy of HEK293T transfected with GFP-Albumin and BFP-CPT2 ( i ) or BFP-Vector ( j ). Representative images were shown. The intensities along the yellow arrows were measured. Scale bars: 5 μm. Data are analyzed by unpaired two-tailed Student’s t test and represented as mean ± SEM. * p < 0.05, ** p < 0.01

Article Snippet: Plasmids pLV-C-GFPSpark-Albumin (HG10968-ACGLN) and pLV-C-GFPSpark-CPT2 (HG18044-ACGLN) were bought from SinoBiological.

Techniques: Western Blot, Co-Immunoprecipitation Assay, Control, Transfection, Confocal Microscopy, Plasmid Preparation, Two Tailed Test

Journal: Signal Transduction and Targeted Therapy

Article Title: Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease

doi: 10.1038/s41392-023-01437-0

Figure Lengend Snippet: Albumosomes regulate CPT2 sorting to mitochondria to maintain mitochondrial homeostasis. a – d Confocal microscopy of HEK293T transfected with GFP-Albumin and BFP-CPT2 ( a ), GFP-Albumin and BFP-Vector ( b ), GFP-Vector and BFP-CPT2 ( c ), GFP-Vector and BFP-Vector ( d ). Mito-tracker was used for mitochondria staining. Representative images were shown. The intensities along the yellow arrows were measured. Scale bars: 5 μm. e HEK293T mitochondria isolation assay. Mitochondria was isolated from HEK293T transfected with GFP-Albumin and BFP-CPT2 or GFP-Vector and BFP-CPT2. Relative intensity was measured from Western blot results ( n = 3). f IF of albumin and mitochondrial folded CPT2 of HepG2-WT and HepG2-AKO. Mito-tracker was used for mitochondria staining. Representative images were shown. Intensities of albumin and folded CPT2 were measured. Scale bars: 5 μm. g Seahorse mitochondrial respiration assay of HepG2-WT and HepG2-AKO overexpressed with GFP-Vector or GFP-CPT2, respectively. Basal respiration, maximal respiration, spare capacity, ATP production, and proton leak were analyzed. Data are analyzed by unpaired two-tailed Student’s t test and represented as mean ± SEM. ns. represents no significance. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Plasmids pLV-C-GFPSpark-Albumin (HG10968-ACGLN) and pLV-C-GFPSpark-CPT2 (HG18044-ACGLN) were bought from SinoBiological.

Techniques: Confocal Microscopy, Transfection, Plasmid Preparation, Staining, Isolation, Western Blot, Respiration Assay, Two Tailed Test

Journal: Signal Transduction and Targeted Therapy

Article Title: Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease

doi: 10.1038/s41392-023-01437-0

Figure Lengend Snippet: Graphic summary of this study. Stresses from aging or HFD stimulate the expression of mitochondrial proteins related with fatty acid β-oxidation and synthesis, TCA cycle, and respiratory chain, etc. In WT livers, excessive pre-folding CPT2 could be trapped by albumosomes in the cytoplasm, relieving the stress of mitochondria and maintaining lipid metabolic homeostasis, which form a virtuous cycle and inhibit fat deposition. However, in AKO livers, such stress-induced excessive pre-folding mitochondrial proteins enter mitochondria more freely lacking the capture by albumosomes, and stimulate mitochondrial overloaded respiration. Continuously, prolonged stresses aggravate mitochondrial exhaustion and result in lipid metabolic disorders, which lead to a vicious cycle and trigger fat deposition

Article Snippet: Plasmids pLV-C-GFPSpark-Albumin (HG10968-ACGLN) and pLV-C-GFPSpark-CPT2 (HG18044-ACGLN) were bought from SinoBiological.

Techniques: Expressing

Journal: Biosensors

Article Title: Impedimetric Detection of Cancer Markers Based on Nanofiber Copolymers

doi: 10.3390/bios14020077

Figure Lengend Snippet: ( A ) Nyquist diagrams of a SA-PEO/FA-modified SPCE obtained with increasing concentrations of FRs by decade from 0.1 pM to 100 nM. The different colors refer to different concentrations of FRs, black is biosensor without FR, red: 0.1 pM, violet: 1 pM, blue: 10 pM, green: 100 pM, yellow: 1 nM, dark red: 10 nM ( B ) Calibration curve corresponding to the average variation of ΔRct/Rct 0 versus the log concentrations of the FRs measured and inset with the linear part. ( C ) Histogram of the interference analysis of the NF biosensor after incubation with10 nM FRs, 100 nM HSA, and a mixture of both proteins 10 nM FRs+ 100 nM HSA. ( D ) Stability analysis of the NF biosensor using [Fe(CN) 6 ] 3− / 4− after storing for two months at 4 °C. EIS was measured at the OCP with a DC of 10 mV and a frequency range of 100 kHz to 0.1 Hz.

Article Snippet: The Human FOLR2 Protein (FRs) > 90% (Mw = 26.5 KDa) and Human Serum Albumin Protein (HSA) > 95% (Mw = 67.4 KDa) were purchased from ACRO Biosystems, Newark, DE, USA.

Techniques: Modification, Incubation